http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01235600-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_630b360599ea5cf4b5bcbc0c6c0a3e2d |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-12 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-76 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-37 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-12 |
| filingDate | 1988-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f6db09310ec9098628b1d780b025dde http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_940a8033a83b2b9b364064583113bdbf |
| publicationDate | 1989-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H01235600-A |
| titleOfInvention | Method for collecting peptide having carboxyl end |
| abstract | PURPOSE: To make it possible to simply collect carboxyl ended peptide, by cutting a peptide bond between amino-ended amino acid residue and adjacent amino acid residue of a specific peptide by an acid treatment. n CONSTITUTION: A desired polypeptide (A) is dissolved in N-ethylmorpholine buffer containing 4W5mol. urea and the resultant solution is added to protease (API) of 1/20W1/2,000mol. based on the component A and the component A is reacted with the protease at pH 6W11 and 4W50°C and peptide bond between lysin residue in the component A and amino acid residue on successive carboxyl end side is specifically cut to afford a peptide mixture (B). Then the component B is reacted with a solid carrier (C) having a functional group such as imide group capable of forming a covalent bond by reacting with a free amino group thereon to give peptide.solid links (D). The trifluoroacetic acid, etc., is added to the component D and the component D is reacted with the acid, etc., under N 2 atmosphere at 20W80°C to subject the component D to cutting treatment to isolate the peptide. Then the peptide is separated, purified and recovered. n COPYRIGHT: (C)1989,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H06172380-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5470703-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009132649-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0552852-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/US-5431882-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H0642937-U |
| priorityDate | 1988-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.