http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01235590-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_81ae2ab45851e706bbc45d391ad59eb8 http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d9cb217f83556ce2a418344c2d485415 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-08 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-02 |
| filingDate | 1988-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_15bc4e22d3d7c60325f2594798884e11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8f0376ea383327295b305f1bd2ec8a9e http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_899b0aee9d918208ce402e5f3c2b770c http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e311520beb22df3e1f4252a5ad9271ef |
| publicationDate | 1989-09-20-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H01235590-A |
| titleOfInvention | Production of erythrocyte derived cu, zn type superoxide dismutase and catalase |
| abstract | PURPOSE: To separate and to purify a large amount of the title superoxide dismutase and catalase simply and in high purity, by treating an hemolyzed solution with an anion exchanger equilibrated with a phosphoric acid buffer solution at a given pH. n CONSTITUTION: A hemolyzed solution of human, etc. is treated with an anion exchanger equilibrated with a phosphoric acid buffer solution at pH 7.2W7.5 and both Cu, Zn type superoxide dismutase (SOD) and catalase are recovered to give a recovered material (A). Then the component A is concentrated, desalted, SOD and catalase as the same fraction are purified by anion exchange chromatography to give a purified fraction (B). Then the component B is passed through a metallic chelating affinity chromatography resin and separated into SOD fraction (C) and a catalase fraction (D). Then the fraction C is purified in the presence of a thiosulfate by chromatography carrying copper, the fraction D is purified chromatography of hydrophobic mutual action to recover erythrocyte derived Cu, Zn type superoxide dismutase and catalase. n COPYRIGHT: (C)1989,JPO&Japio |
| priorityDate | 1988-03-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 225.