http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01230522-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_382ccfc2ebebcaee15aa7cda4cee86bc |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K14-495 |
| classificationIPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12R1-91 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/A61K35-14 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K14-495 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P21-00 |
| filingDate | 1989-01-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_2b4f05eefa175e71939b90bdeb07c9af http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b04aa49811e47c3d221c72d30cca279 |
| publicationDate | 1989-09-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H01230522-A |
| titleOfInvention | Purification of transforming growth factor-beta |
| abstract | PURPOSE: To more rapidly purify in high yield with the minimum number of operations, by extracting blood platelet with an acid-ethanol and carrying out the cation-exchange and hydrophobic separations of the obtained extract. n CONSTITUTION: 100-500 unit, preferably 100-300 unit blood platelet is extracted with an acid-ethanol with <4.0, preferably 2.0-3.5pH and ≥50%(V/V), preferably 70-80%(V/V) ethanol concentration. The obtained extract is adjusted to the pH (about 5.5) of a buffer solution used for equilibration of a cation-exchange resin and subjected to the cationic-exchange separation to isolate a fraction having a transforming growth factor -β (TGF-β) activity. The resultant fraction is subjected to the hydrophobic separation to provide purified TGF-β. Purified TGF-β can be also obtained by the two step separation of a conditioned medium containing TGF-β obtained by growing human melanoma cell line M3827 (ATCC CRL-9193) or normal rat renal cell line NRK-49F (ATCC CRL-1570). n COPYRIGHT: (C)1989,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/WO-9961474-A1 |
| priorityDate | 1988-01-07-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.