http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01227963-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_01f4342d9c3f01567ee3e9084a88230c |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-54326 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-743 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-585 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-58 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-53 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-74 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-543 |
| filingDate | 1989-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_53c4e43b690fa77c9512f0272b1a8f5c |
| publicationDate | 1989-09-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H01227963-A |
| titleOfInvention | Method and kit for immunoassay |
| abstract | PURPOSE: To decide whether an objective substance is present more than a threshold quantity by causing a sample to react with a fourth labeled substance after a known quantity of second substance specific to the objective substance to be detected and a third substance exhibiting reactivity identical to that of the objective substance are brought into reaction with the sample. n CONSTITUTION: Pregnanediol-3-glucuronide (P 3 G) is caused to be bonded covalently with gelatin and gelatin-P 3 G is adsorbed to a well of microtiter plate. The well is added with a sample solution and a solution containing a known quantity of anti-P 3 G antibody in PBS before being incubated. The solution is then decanted from the well and the plate is cleaned. Subsequently, it is added with a solution containing anti-P 3 G/horseradish peroxidase bond, incubated and cleaned. It is further added with a solution of coloring substrate containing tetramethylbenzidine and hydrogen peroxide and the intensity of coloring is measured. A definite coloring takes place when a specified quantity or more of P 3 G is present in the sample. n COPYRIGHT: (C)1989,JPO |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H03503215-A http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2009168495-A |
| priorityDate | 1988-02-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 32.