http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01152352-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2b30aaab6b2cd23887e078e5c256e027 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-447 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N33-50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N21-33 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G01N27-26 |
| filingDate | 1987-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9b5e49487c9c25e62270877172eaa8be |
| publicationDate | 1989-06-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-H01152352-A |
| titleOfInvention | Detection of mutagen |
| abstract | PURPOSE: To enable qualitative or quantitative detection of a mutagen quickly, by having the mutagen adsorbed by DNA to be irradiated with ultraviolet rays after the application therefor of a gel electrophoresis. n CONSTITUTION: A mutagen such as nitrosoguanidine, benzpyrene, ethylmethane sulfonic acid or nitrous acid is adsorbed by DNA. To achieve the adsorption, solutions, one with a DNA density of 5W10μg/ml and the other with a mutagen density of about 10W20mM are mixed for about 5W10min under a room temperature condition. In this case, a solvent employs, for example, a TE buffer or the like. Then, a gel such as agarose, dexitrane, polyacrylamide or a crosslinked bond thereof is used at a density of about 0.3W1% and an electrophoresis is performed under conditions of a voltage of about 50W100V and required time of about 30W60min. Thereafter, when the solution from which the agarose gel was separated is irradiated with ultraviolet rays, light is emitted to measure absorbance thereof thereby enabling quantitative detection from a calibration curve. n COPYRIGHT: (C)1989,JPO&Japio |
| isCitedBy | http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01291145-A |
| priorityDate | 1987-12-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 22.