http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-H01132386-A

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http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-81
filingDate 1987-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
inventor http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_9c61d365337d1ed3194713a354fa24a7
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cf04c04fc11228dada12e681470f86f4
publicationDate 1989-05-24-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H01132386-A
titleOfInvention Plasmid and production thereof
abstract PURPOSE:To obtain a plasmid including autoreproduction of Saccharomycopsis lipolytica, by separating a plasmid from a leucine-demanding Saccharomyces cerevisiae transformed with a gene library. CONSTITUTION:An ampicillin-resistant plasmid is scissored with a restriction enzyme, Leu 2 gene range of Saccharomycopsis lipolytica is isolated, Escherichia coli vector is scissored with a restriction enzyme and connected to the isolated range. The gene library of Saccharomycopsis lipolytica is reproduced by using the connected plasmid. Then leucine-demanding Saccharomycopsis lipolytica as a host is transformed with the gene library. A plasmid is separated from the prepared transformant, Escherichia coli is transformed and the ampicillin- resistant plasmid is separated.
priorityDate 1987-11-19-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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Total number of triples: 24.