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filingDate 1987-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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publicationDate 1989-05-01-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-H01112992-A
titleOfInvention Production of l-isoleucine
abstract PURPOSE: To industrially and advantageously obtain the titled compound useful as a feed additive, etc., by enzymically reacting α-ketobutyric acids with pyruvic acids in the presence of microbial cells of the genus Brevibacterium requiring biotin in an aqueous solution containing ethanol. n CONSTITUTION: α-Ketobutyric acid or a salt thereof is reacted with pyruvic acid or a salt thereof in the presence of microbial cells [e.g. Brevibacterium flavum MJ-233 (FERM-P No.3068)] belonging to the genus Brevibacterium requiring biotin in an aqueous solution containing at least ethanol to produce L- isoleucine in the solution. The resultant reaction solution is then centrifuged to separate the microbial cells and collect a supernatant, which is subsequently passed through a strong acidic cation exchange column to elute an adsorbed substance. Thereby the aimed L-isoleucine is obtained. n COPYRIGHT: (C)1989,JPO&Japio
priorityDate 1987-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date>
type http://data.epo.org/linked-data/def/patent/Publication

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