| abstract |
An object of the present invention is to provide novel thermostable DNA polymerase variants with reverse transcription activity. According to the present invention, it has activity to catalyze the reverse transcription reaction in the presence of Mg 2+ , has an identity of 90% or more with the amino acid sequence set forth in SEQ ID NO: 5, and both positions 744 and 745 are positively charged. A DNA polymerase is provided comprising an amino acid sequence substituted with an amino acid having |