http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-6850402-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-158 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6848 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6883 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6883 |
| filingDate | 2020-07-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2021-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2021-03-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-6850402-B1 |
| titleOfInvention | Method for detecting or quantifying the SMN1 gene |
| abstract | The present invention provides a primer for detecting and quantifying a homozygous deletion of the SMN1 gene, which is the causative gene of SMA, using dried filter paper blood, and a method for specific detection and quantification of the SMN1 gene using the primer. The purpose is. The present invention is a method for detecting the SMN1 gene in dried filter paper blood by real-time PCR, which comprises the following steps (A) to (D). (A) A step of adding the dried filter paper blood to a PCR reaction tube (B) A step of adding a PCR reagent to a PCR reaction tube, in which at least 1% of the SMN1 gene is reacted with the PCR reagent. Step (C) PCR reaction including the primer, polymerase, dNTP and intercalator or fluorescently labeled probe designed to be less than, and step (D) PCR reaction in which the PCR reagent and the dried filter paper blood are added. The step of optically detecting the target nucleic acid in the SMN1 gene amplified by |
| priorityDate | 2019-07-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 27.