http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-5216943-B1
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/Y10T436-143333 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6809 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6816 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N21-6486 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate | 2012-06-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| grantDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2013-06-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-5216943-B1 |
| titleOfInvention | Method for detecting G-quadruplex formation |
| abstract | The method of the present invention aims to specifically detect G-quadruplex even in the presence of potassium ions. The method of the present invention uses the phenomenon that thioflavine T emits strong fluorescence when reacted with G-quadruplex in the presence of potassium ions, and determines whether the target DNA forms G-quadruplex. In the method of the present invention, a first sample solution containing potassium ions and target DNA is maintained under conditions where G-quadruplex is formed, and then thioflavin T is added to measure the first fluorescence intensity value. The second sample solution containing the target DNA is maintained under conditions where G-quadruplex is destabilized, and thioflavin T is added to measure the second fluorescence intensity value. If the difference between these values is 0 or more It is determined that the target DNA can form a G-quadruplex. |
| priorityDate | 2011-08-11-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 37.