http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-4776133-B2
Outgoing Links
Predicate | Object |
---|---|
classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-68 |
filingDate | 2001-01-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
grantDate | 2011-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationDate | 2011-09-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-4776133-B2 |
titleOfInvention | Isolation of nucleic acids from stool samples and other biological materials rich in inhibitors |
abstract | The invention related to a method for the stabilization, purification or/and isolation of nucleic acids from material samples, in particular, stool samples, which can contain impurities and inhibitors or interfering substances. The invention further relates to a reagent kit for carrying out this method. The basis of the invention is, in particular, a method for purification, stabilization or/and isolation of nucleic acids from material samples, whereby a buffer is added to the sample containing the nucleic acids, with a pH value of 2 to 7, a salt concentration of at least 100 mM, or/and a phenol neutralizing substance. According to the invention, pure nucleic acids which may be amplified can be obtained from faecal samples by a simple method, which are suitable for diagnostic proof of infection, in particular, bacterial or viral infection, or mutation, in particular, for tumor-specific DNA mutations. |
priorityDate | 2000-02-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 51.