| abstract |
The present invention relates to methods of conducting biochemical reactions, such as in aqueous in vitro reaction systems. This method involves macromolecules, particularly polypeptides, that contain one or more functional naturally occurring modified regions (IDRs). The present invention also relates to IDR-macromolecules, including IDR-polypeptides, including macromolecules or polypeptides comprising tagged amino acid sequences comprising or consisting of one or more functional IDRs. Such functional IDRs can increase the efficiency of biochemical reactions. The present invention relates to kits containing any such macromolecules and polypeptides. The invention further uses any such macromolecules and polypeptides, including in combination with polyvalent metal ions, to stimulate or enhance liquid-liquid demixing in solution, thereby increasing the efficiency of biochemical reactions. It relates to a method for providing a reagent capable of enhancing |