http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022525373-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1093 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6844 |
| filingDate | 2019-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-05-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2022525373-A |
| titleOfInvention | Methods, systems and applications for constructing sequencing libraries based on target regions of methylated DNA |
| abstract | Provided are methods, systems and applications for constructing sequencing libraries based on target regions of methylated DNA. The method is a step of obtaining a sample of DNA with a universal sequence converted with hydrogen bicarbonate, and a step of amplifying using a first specific primer and a first universal primer. The specific primer is located upstream of the target region, the first universal primer is a step that matches or overlaps at least part of the universal sequence, and the second specific primer, the second universal primer and the label primer. The second specific primer is located downstream of the first specific primer and upstream of the target region, and the second universal primer is the second, in the step of using and amplifying to obtain a sequencing library. The label primer overlaps a part of the sequence of the first universal primer, or the second specific primer is located downstream of the target region and overlaps with at least a part of the sequence of the specific primer of the second universal primer. Includes a step that overlaps at least a portion of the sequence of the first specific primer and the label primer overlaps a portion of the sequence of the second specific primer. |
| priorityDate | 2019-05-21-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 31.