http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022520283-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2800-90 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87 |
| filingDate | 2020-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2022-03-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2022520283-A |
| titleOfInvention | Cell-permeable transposase |
| abstract | Sleeping Beauty (SB) transposons are an efficient non-viral tool for inserting transgenes into cells. Its widespread use in gene therapy has been hampered by the unregulated transposase gene activity and the inability to use the transposase protein directly. According to the present invention, SB transposase can spontaneously permeate mammalian cells and be delivered together with transposon DNA to genetically modify various cell lines, embryonic stem cells, hematopoietic stem cells, and induced pluripotent stem cells. It is related to the findings. The present invention provides methods and compounds for applying the cell permeation function of transposases in methods of genetic manipulation of cells, as well as methods and compounds for using transposases as shuttles to deliver cargo within or even within target cell organelles. offer. The techniques of the invention can be used to regulate the frequency of genomic integration, which adds an additional hierarchy of safety, opening up opportunities for advanced utilization in genetic engineering and gene therapy. [Selection diagram] None |
| priorityDate | 2019-02-19-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 171.