http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2022163885-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_a6638ac6b1020cd2c62d09d748bc1ab9 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-11 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
filingDate | 2021-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fb46506e1a18ab37d0c7a3113d84422a http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_55b75cd7fd61cb3a89c287da41e9dc7b |
publicationDate | 2022-10-27-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2022163885-A |
titleOfInvention | Screening method for substances involved in collagen biosynthesis and/or post-biosynthetic processes |
abstract | An object of the present invention is to provide a screening method for agents involved in collagen biosynthesis and/or post-biosynthetic processes. The object is to encode a fusion protein of (1) a collagen-related protein, which is a preprocollagen protein, a procollagen protein, a collagen protein, or a variant thereof, and a fluorescent protein or a luminescent protein of the present invention. A step of contacting a cell into which a collagen promoter vector containing a nucleic acid and a collagen promoter has been introduced with a test substance, wherein fusion of a fluorescent protein or a luminescent protein is inserted from the C-terminus of the collagen protein to the N-terminus of 30 amino acids. , an insertion from the N-terminus to the C-terminus of 30 amino acids from the C-propeptide, and an insertion into the N-propeptide, and (2 ) measuring the fluorescence or luminescence in the cell culture supernatant and/or in the cells. [Selection figure] None |
priorityDate | 2021-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 64.