| Predicate |
Object |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2537-164
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2525-186
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01026
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-154 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-1051
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-0071
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6876
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6827
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01026
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y204-01027
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-12
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P17-16 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2019-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate |
2021-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2021509587-A |
| titleOfInvention |
Cytosine-modified, bisulfite-free, base resolution identification |
| abstract |
The present disclosure provides a method for identifying bisulfite-free in nucleic acid sequences at the positions of 5-methylcytosine, 5-hydroxymethylcytosine, 5-carboxylcytosine and 5-formylcytosine. The methods described herein provide DNA or RNA methylation and hydroxymethylation analysis with a mild reaction that quantitatively detects modified cytosines at base resolution without affecting unmodified cytosines. Combining TET oxidation and reduction with borane derivatives (eg, pyridineborane and 2-picolinborane (pic-BH 3)), referred to herein as TAPS (TET Assisted Pyridine borane Sequanting). Provided herein with new methods for identifying 5 mC and 5 hmC (Table 1). [Selection diagram] Fig. 1 |
| priorityDate |
2018-01-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |