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filingDate 2019-09-10-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2021-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2021509285-A
titleOfInvention Streptomyces sericolor mutant strain, beta-agarase production method using this, and neo-agarooligosaccharide production method using this
abstract In the present invention, the base sequence of the DagB gene present in the wild-type Streptomyces coelicor A3 (2) strain is point-mutated by UV irradiation, and the Streptomyces coelicor A3 (2) _M22- A 2C43 strain is provided. The Streptomyces coelicol A3 (2) _M22-2C43 strain according to the present invention expresses a DagB mutant enzyme that hardly expresses DagB beta-agarase or has almost no beta-agarase activity. It is not necessary to separately purify the DagA enzyme from the agar or agarose using the culture solution of the Streptomyces coelicol A3 (2) _M22-2C43 strain or the supernatant thereof. It is possible to produce a neo-agarooligosaccharide having a relatively high content of neoagarotetraose or neoagarohexaose from neoagarobiose).
priorityDate 2018-08-08-04:00^^<http://www.w3.org/2001/XMLSchema#date>
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