| abstract |
PROBLEM TO BE SOLVED: To provide a non-shared complex of an aptamer and a target, and a method for producing and using the complex. A non-covalent complex of an aptamer and a target in which one, some or all of the pyrimidines in the aptamer's nucleic acid sequence are modified at the 5-position and the modifications are benzyl, isobutyl, indole, or naphthyl. The complex, wherein the dissociation rate (t 1/2 ) of the aptamer from the target is greater than or equal to 30 minutes and the aptamer has a dissociation constant (K d) of about 1 μM or less relative to the target. Provided are methods of producing and using the complex. [Selection diagram] None |