| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_2a819eda0adf22936a52362eeebb9fb4 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B82Y5-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2510-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-20
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N2310-10 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-0647
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/A61K35-17
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-102
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-113
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1138
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-907 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N9-22
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-87
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-62
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-28
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K19-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K16-46
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-13
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-09 |
| filingDate |
2021-05-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e60dd2e57fd9dc88099d408f7c086a8d
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_56256f35480ae9ff4d865ab00e819b81
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fae3c4f4f5ad7a4954ad4bc997cfc416
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_503d81ac666672fee4580cb513982736
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_fd1f4b68037223d61b53a07300577855 |
| publicationDate |
2021-08-26-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2021121202-A |
| titleOfInvention |
Protein delivery in primary hematopoietic cells |
| abstract |
PROBLEM TO BE SOLVED: To provide a method and composition for highly efficient delivery of Cas9 and Cas9 ribonucleoprotein to cells including primary hematopoietic cells and primary hematopoietic stem cells. A) A step of providing a reaction mixture containing a Cas9 ribonucleoprotein complex and a cell, wherein the Cas9 ribonucleoprotein complex is specific to the Cas9 nuclease domain and the target region of the cell genome. An in vitro or exvivo method for editing the cell's genome, comprising stepping with a guiding RNA to hybridize; and b) introducing the Cas9 ribonucleoprotein complex into the cell by electroporation. The method in which the cells are primary hematopoietic cells or primary hematopoietic stem cells. [Selection diagram] FIG. 1A |
| priorityDate |
2015-01-30-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |