| abstract |
PROBLEM TO BE SOLVED: To modify and improve the structural and biophysical properties of an immunobinder, particularly a single chain antibody (scFv), such as stability, solubility and / or antigen-binding affinity, for sequence-based analysis and rationalization. Provides a way to use a typical strategy. For substitutions in a method of engineering an immunobinder, particularly scFv, by making one or more substitutions at amino acid positions determined by analysis of a database of selected stable scFv sequences. This is the method in which a preferable amino acid residue has been determined. [Selection diagram] None |