http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021058143-A
Outgoing Links
Predicate | Object |
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assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bfdf49224a405be1f7b653c56ebad94 |
classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-53 |
filingDate | 2019-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_941c56863bb98093fc57fd2c504cc8f1 |
publicationDate | 2021-04-15-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
publicationNumber | JP-2021058143-A |
titleOfInvention | Method for detecting primers and GAPDH mRNA |
abstract | PROBLEM TO BE SOLVED: To provide a method for amplifying and detecting GAPDH mRNA present in a sample with high sensitivity, rapidly and specifically. A first primer having a sequence complementary to the 3'end of a specific base sequence of GAPDH mRNA contained in a sample and a second primer having a sequence homologous to the 5'end of the specific base sequence. A primer set for amplifying a nucleic acid containing the specific base sequence or a complementary sequence of the specific base sequence, which comprises two primers, and the first primer is one of SEQ ID NOs: 1, 5 and 9. It is an oligonucleotide that can specifically hybridize with the nucleotide sequence described above under stringent conditions, and the second primer can specifically hybridize with the nucleotide sequence set forth in SEQ ID NO: 14 or 20 under stringent conditions. GAPDH mRNA is detected using the above-mentioned primer set, which is an oligonucleotide. [Selection diagram] None |
priorityDate | 2019-10-08-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 206.