http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021048883-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_37c1e47d1f3818d1fb3806d27d2fb427 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1027 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1031 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P19-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6844 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12P19-34 |
| filingDate | 2021-01-05-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_b72d233655f3122e81ca6ad81ac365d7 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3c9b36e80f0d63044a41fe4229a8d540 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_235ee72afd8a2a7b9eeb5fd5b2f2e846 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c5e6fa0c506e012b3594e010f4136725 |
| publicationDate | 2021-04-01-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2021048883-A |
| titleOfInvention | Assembly of nucleic acid molecules via PEG |
| abstract | PROBLEM TO BE SOLVED: To provide a method for assembling a nucleic acid molecule from a set of overlapping oligonucleotides. A method comprising contacting a set of overlapping oligonucleotides with a DNA polymerase, a dNTP mixture, and a condensing agent to form an assembly mixture. In one embodiment, the condensing agent is polyethylene glycol (PEG). The presence of the condensing agent facilitates the nucleic acid assembly process of the present invention. The assembly mixture is then subjected to multiple cycles, each cycle containing an annealing step, an extension step, and a denaturation step, whereby the desired nucleic acid molecule is assembled. In some embodiments, one or more of the steps vary in time. [Selection diagram] None |
| priorityDate | 2012-12-13-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 229.