http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2021020958-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6a07cc4bec41fe70ec992c1f8f961242 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Y304-21062 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-026 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-54 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12P21-02 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K11-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-02 |
| filingDate | 2020-10-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5a60142fa68ea5683c82039c6be7ea72 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bc6ec6ee16f75cc50d8327e16c67327d http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_bd7d66a3a561feec26a3dccf98ce4c7f http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_027812f25f8110e0e318fc9962374295 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_c7d55727c4692f536f5ad469e36bbf62 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_cac1480c80b79417c43131c48d328fd0 |
| publicationDate | 2021-02-18-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2021020958-A |
| titleOfInvention | Peptide fragment condensation and cyclization using subtilisin mutants with improved ratio of synthesis to hydrolysis |
| abstract | PROBLEM TO BE SOLVED: To develop a method for enzymatically synthesizing a (oligo) peptide. SOLUTION: The method of the present invention comprises coupling (a) a (oligo) peptide C-terminal ester or thioester with (b) an (oligo) peptide nucleophilic reagent having an N-terminal unprotected amine, the coupling comprising water. Performed in the containing fluid, the coupling was performed as compared to the subtilisin BPN' or its homologous sequence represented by SEQ ID NO: 2, the following mutations: -Amino acid deletion corresponding to positions 75-83,- By a mutation at the amino acid position corresponding to S221, a mutation that is S221C or S221 selenocysteine-preferably by a subtilisin BPN'mutant or a homologue thereof, including a mutation at the amino acid position corresponding to P225. Catalyzed, the amino acid position is defined according to the sequence of subtilisin BPN'represented by SEQ ID NO: 2. [Selection diagram] FIG. 1A |
| priorityDate | 2014-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
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