| abstract |
PROBLEM TO BE SOLVED: To provide a manipulated, living, three-dimensional liver tissue composition. A method of constructing a living, three-dimensional liver tissue construct, a. The step of preparing one or more aggregated multicellular aggregates containing mammalian hepatocytes, b. The one or more aggregated multicellular aggregates are placed on the support to i. At least one layer containing multiple cell types, spatially arranged relative to each other to create a planar structure, and ii. A step of forming at least one of a plurality of layers in which at least one layer is compositionally or structurally different from at least one other layer in order to form a layered structure, as well as c. Incubating one or more of the multicellular aggregates for a period of about 1 hour to about 30 days to allow them to aggregate and form a living, three-dimensional liver tissue construct. Methods, including steps to enable. [Selection diagram] FIG. 8 |