| abstract |
The present specification relates to transgenic animals and transgenic embryos that produce components of modified nucleases. According to the disclosure herein, a transgenic animal (or embryo) that produces a component of a modified nuclease is a first cell having a genome containing a first toolbox and a second having a genome containing a second toolbox. A transgenic animal (or genome) with a cell, the first toolbox and the second toolbox are a polynucleotide encoding an RNA-inducible endonuclease, and a guide nucleic acid that can specifically bind to a target site, respectively. The first toolbox resides at the first locus of the first cell genome and the second toolbox resides at the second locus of the second cell genome, comprising at least one of the polynucleotides encoding However, the first locus is different from the second locus. |