| abstract |
Provided is a method of purifying sulfatase using metal chelate chromatography without the use of tags (eg, His-tag, etc.). In one embodiment, the method for purifying sulfatase comprises (a) providing a sulfatase-containing solution containing one or more impurities and (b) using a metal affinity chromatography resin to prepare the sulfatase-containing solution. One step of performing chromatographic separation, (c) a step of performing a second chromatographic separation using a cation exchange chromatography resin, and (d) a final chromatographic separation using an anion exchange chromatography resin. The step of removing the impurities is included. |