abstract |
The present invention relates to glycosyltransferases, mutants and uses thereof. Specifically, a method for glycosylation in vitro, which comprises transferring a glycosyl group from a sugar donor to a C-3 hydroxyl group of a tetracyclotriterpene compound in the presence of a glycosyltransferase, To form a tetracyclotriterpene compound, wherein the glycosyltransferase is a glycosyltransferase represented by SEQ ID NO: 4 or a derivative polypeptide thereof, or a glycosyltransferase represented by SEQ ID NO: 4 or a derivative thereof. Methods are provided that are derivative polypeptides. |