| abstract |
The present invention provides engineered penicillin G acylase (PGA) enzymes, polynucleotides encoding the enzymes, compositions containing the enzymes, and methods of using engineered PGA enzymes. The present invention also provides an acylated insulin composition produced using at least one engineered penicillin G acylase provided herein. In some further embodiments, the invention provides a composition comprising an acylated insulin produced using at least one method provided herein. |