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filingDate 2018-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-04-23-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2020512539-A
titleOfInvention Method for adapting UV cell optical path length in a chromatography system
abstract The present invention is a method for determining the operating state of a chromatography column (1; 39,47,59; 107,109,111,113), wherein the feed representing the composition of the feed liquid material fed to the inlet of the column. The step of detecting the liquid signal (21; 201), the step of detecting the UV absorbance in the feed material, and the eluate signal (23; 203, 205, 207, 209) representing the composition of the eluent from the column is detected. And using the feed and eluate signals to determine the operating state of the column. The feed signal is generated using a first UV detector having a first UV cell pathlength operating at a first UV wavelength and the eluent signal is generated at a second UV wavelength operating at a second UV wavelength. Generated using a second UV detector having a UV cell path length of The method comprises the steps of determining a first threshold value based on the detected UV absorbance in the feed material, and determining the first UV cell optical path length and / or the first UV wavelength based on the first threshold value. And a step of selecting.
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