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filingDate 2018-03-06-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationDate 2020-03-12-04:00^^<http://www.w3.org/2001/XMLSchema#date>
publicationNumber JP-2020507758-A
titleOfInvention Single light source, two optical channel sequencing
abstract A system for determining the nucleotide sequence of a polynucleotide is disclosed. The system can include a light source (eg, a laser or an LED) configured to generate light of a predetermined wavelength. The detector of the system can detect the fluorescence emission at the first wavelength and the second wavelength. The processor of the system identifies the nucleotide as a first type if the fluorescence is not detected by the at least one detector, and if the fluorescence at the first wavelength of light is detected by the at least one detector. Identifying the nucleotide as a second type and identifying the nucleotide as a third type if the fluorescence emission at the second wavelength of light is detected by the at least one detector; Nucleotides are identified as a fourth type when fluorescence emission at the wavelength and the second wavelength is detected by at least one detector.
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