| abstract |
Disclosed are methods, devices, and systems for analyte analysis using nanopores. The methods, devices, and systems use first and second binding members, each of which specifically binds an analyte in a biological sample. The method further includes detecting and / or counting the cleavable tag attached to the second binding member, and correlating the presence and / or number of the tag with the presence and / or concentration of the analyte. . Detection and / or counting may be performed by translocating the tag / second binding member through the nanopore. Also provided herein is an instrument that includes an array of electrodes for actuating droplets and is further programmed to operate a cartridge that includes an electrochemical species sensing area. Using the device to analyze a sample in a cartridge comprising an array of electrodes for actuating droplets and further comprising a nanopore layer for detecting tag / second binding member translocation through the nanopore. Can be. [Selection diagram] FIG. 1A |