| abstract |
The present invention relates to a stable method for introducing at least one induction cassette into a cell and enabling controllable transcription from within the induction cassette. The method can be used on any cell type from any eukaryote, but in introducing an induction cassette into a pluripotent stem cell, such as an animal or human pluripotent stem cell (hPSC). Has a specific application. The induction cassette is controllably inserted to ensure that the genetic material it contains is not silenced or negatively affected from the insertion site and that transcription of the genetic material is controlled. . [Selection diagram] FIG. |