| abstract |
The variant of EndoS2 has one or more mutations in the sequence of wild-type EndoS2 (SEQ ID NO: 1), and the one or more mutations are residues 133-143, residues 177-182, residues 184-189, In the peptide region located within residues 221-231 and / or residues 227-237, the variant of EndoS2 has a lower hydrolytic activity than wild-type EndoS2, and transglycosylation. Activity is higher than wild-type EndoS2. A method for preparing an engineered glycoprotein using an EndoS2 mutant involves coupling an activated oligosaccharide to a glycoprotein acceptor. The activated oligosaccharide is glycan oxazoline. [Selection diagram] FIG. |