abstract |
PROBLEM TO BE SOLVED: To provide a serum-free culture method for human chondrocytes, which can be effectively cultured and obtain good transplantation results, and a serum-free medium which can be used for the method. SOLUTION: An enzyme treatment step of treating a tissue containing human cartilage cells with a proteolytic enzyme, an inhibitor treatment step of treating the tissue with an inhibitor of the proteolytic enzyme after the enzyme treatment step, and an inhibitor. A serum-free culture method for cartilage cells, comprising a culture step of culturing the tissue in a serum-free medium containing ITS, FGF2, and hydrocortisone with either or both of cultogenin and SAG after the treatment step. Serum-free medium for chondrocyte culture containing ITS, FGF2, and hydrocortisone with either or both of cartogenin and SAG. [Selection diagram] Fig. 1 |