http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020191783-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_d80f1040809503e54509c871ba828f75 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12M3-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-04 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-0735 |
| filingDate | 2017-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_4f1923d277ab99012ce65acdc55b2130 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_1661bc83037549d717eaa074d322d101 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_f382f67b78e919e37a48cba5fd59cf50 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_3f7955fcaeff442876ecdf4ce4970986 |
| publicationDate | 2020-12-03-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2020191783-A |
| titleOfInvention | Position-specific determination method for undifferentiated state of pluripotent stem cells by culture medium analysis |
| abstract | PROBLEM TO BE SOLVED: To detect a differentiated state of pluripotent stem cells in a culture vessel in a position-specific manner with high sensitivity, and to culture pluripotent stem cells in an undifferentiated state, without the judgment of a skilled engineer. To provide a cell culture device for. A method for determining the undifferentiated state of pluripotent stem cells cultured in a cell culture vessel in a position-specific manner, wherein the pluripotent stem cells are cultured and filled with a culture medium. And, a cell culture container having an inflow port and an outflow port arranged at both ends of the flow path is prepared, and the culture medium in the cell culture container is discharged from the outlet, and the culture medium for each predetermined outflow amount is prepared. Measure both the physical properties and the total outflow of the culture medium, The undifferentiated state of the pluripotent stem cells was determined based on the physical properties of the culture medium for each predetermined outflow amount, and the cell culture of the pluripotent stem cells determined to be in the undifferentiated state based on the total outflow amount of the culture medium. A method is used that comprises determining the position of the container on the flow path. [Selection diagram] FIG. 9 |
| priorityDate | 2017-08-17-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 167.