| Predicate |
Object |
| assignee |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_8e3618a9ffc4a964edbaa07136414a16 |
| classificationCPCAdditional |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2600-166
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q2545-101 |
| classificationCPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1065
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-10
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G16B30-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-689
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6874
http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 |
| classificationIPCInventive |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-06
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12M1-00
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6888
http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/G16B30-10 |
| filingDate |
2020-07-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor |
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_e777aed2d5985cb81be38791f6f832f1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6ea9a4e21cf8bf0a8d8f0c941425ff37
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_595ae8e8c6840811d10e41204d5af250
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0c74f1b382f9babfce456b52c0aa893c
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_aaaaea3c0217a8490e62570b0f3c3ec1
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_0293da733206a5be53c32c9d196dbb01
http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_6a220a842e1408bc4230bce962e8f003 |
| publicationDate |
2020-11-12-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber |
JP-2020182491-A |
| titleOfInvention |
Synthetic nucleic acid spike-in |
| abstract |
PROBLEM TO BE SOLVED: To provide a method for determining the relative abundance of one or more non-host species in a sample from a host. SOLUTION: A starting amount of at least 1,000 synthetic nucleic acids containing a unique variable region is added to a sample, and (b) a sequencing assay is performed on a part of the target nucleic acid and a part of the synthetic nucleic acid in the sample. To obtain a target and synthetic nucleic acid sequence read containing a unique variable region sequence, and (c) (i) quantify the number of different variable region sequences in the synthetic nucleic acid sequence read to obtain a unique sequence determination value. Obtained, (ii) the starting amount of the synthetic nucleic acid is compared with the unique sequencing value described above to detect a decrease in the variety of the synthetic nucleic acid, and (d) the decrease in the variety of the synthetic nucleic acid is used to use the target nucleic acid in the initial sample. A method for determining the abundance of a target nucleic acid, which comprises calculating the abundance of the target nucleic acid. [Selection diagram] Fig. 5 |
| priorityDate |
2016-03-25-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type |
http://data.epo.org/linked-data/def/patent/Publication |