| abstract |
PROBLEM TO BE SOLVED: To provide a method for producing a multi-dose antigen pulsed dendritic cell vaccine for injection. Contacting dendritic cells (DCs) with at least one antigen, activating DCs with at least one TLR agonist, DCs in multiple doses, including initial immunization doses and multiple booster doses. A method of producing an effective amount of at least one cytokine to generate a T cell response when the DC is thawed, comprising cryopreserving in. A method further comprising thawing a DC, in which the DC produces an effective amount of at least one cytokine to generate a T cell response. [Selection diagram] Fig. 5 |