| abstract |
A method for producing retinal cells or retinal tissue from pluripotent stem cells. SOLUTION: The cell aggregate containing Oct3/4 positive cells and cells expressing one or more selected from the group consisting of Sox2, Sox1, Pax6 and Otx2, comprising the following steps (1) and (2): Production method: (1) The first step of culturing human pluripotent stem cells in a medium containing an undifferentiated maintenance factor in the absence of feeder cells, (2) the pluripotent stem cells obtained in the first step, The second step of forming cell aggregates by suspension culture in the presence of a substance that acts on the sonic hedgehog signaling pathway. [Selection diagram] None |