| abstract |
PROBLEM TO BE SOLVED: To provide a composition and a method for biosynthesis of 1,4-butanediol and its precursor. The present invention provides at least one exogenous enzyme encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthase, CoA-dependent succinate semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase. Provided are non-naturally occurring microbial biocatalysts, including microorganisms having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway with nucleic acids, wherein the exogenous nucleic acid comprises monomeric 4-hydroxybutanoic acid (4 -Expressed in an amount sufficient to produce HB). [Selection diagram] None |