http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020054355-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_0471e47f3a2760e9befddc375477afa7 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-00 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K16-005 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N15-1082 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6845 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-6854 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-686 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6806 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5023 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-5047 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N15-10 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C40B40-02 |
| filingDate | 2019-11-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_8a702af12dff8d24d21d767ae588b2d6 http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_5c9cedb20fd853ea20c40641d8074fda |
| publicationDate | 2020-04-09-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2020054355-A |
| titleOfInvention | Method for recovering two or more genes or gene products encoding immune receptors |
| abstract | The present invention provides a method for recovering two or more genes or gene products encoding immune receptors, a method for preparing a library of expression cells, and a method for screening the library. Kind Code: A1 Two or more genes or gene products are contained in a predetermined source cell and include the following steps. a) encapsulating the source cells in a microreactor with the mRNA capture site; b) lysing the cells in the microreactor, releasing cellular mRNA into the lumen of the microreactor and binding it to the mRNA capture site C) reverse transcription of the mRNA bound to the mRNA capture site to obtain a corresponding cDNA gene product; d) preparing a construct containing two or more gene products encoding subunits of an immunoreceptor; And e) cloning the PCR product into a plasmid vector to obtain a bicistronic or polycistronic expression construct. [Selection diagram] None |
| priorityDate | 2014-02-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 169.