abstract |
An object of the present invention is to provide a method for producing a target protein using a recombinant cell that autolyses by external stimulus, a method for lysing the recombinant cell, and the recombinant cell. A step of lysing a recombinant cell expressing a target protein and a polypeptide having a bacteriolytic activity by physically or chemically externally stimulating the recombinant cell, and the target protein Recovering, wherein the expression of the polypeptide is controlled by a constitutive promoter, and the polypeptide is expressed in the cytoplasm of the recombinant cell. [Selection diagram] None |