| abstract |
An object of the present invention is to provide a modified Cas9 protein in which the restriction of a target sequence is relaxed while maintaining the binding ability to a guide RNA, and a use thereof. In the Cas9 protein, arginine at position 1335 is alanine (R1335A), isoleucine (R1335I), methionine (R1335M), threonine (R1335T) or valine (R1335V), and leucine at position 1111 is arginine (L1111R), 1135. Position aspartic acid to valine (D1135V), position 1218 to arginine (G1218R), position 1219 to phenylalanine (E1219F), position 1322 to arginine (A1322R), and position 1337 to arginine. And a protein containing an amino acid sequence mutated to (T1337R). [Selection diagram] None |