abstract |
A method for obtaining a microbial oil comprising one or more polyunsaturated fatty acids (PUFA) from one or more microbial cells. The method comprises the steps of: (a) lysing cells containing microbial oil to form a lysed cell composition; (b) demulsifying the lysed cell composition, comprising the step of adding at least one enzyme. A method is disclosed that includes forming an emulsified lysed cell composition, (c) separating oil from the demulsified lysed cell composition, and (d) recovering the oil. [Selection diagram] None |