http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2020018193-A
Outgoing Links
| Predicate | Object |
|---|---|
| assignee | http://rdf.ncbi.nlm.nih.gov/pubchem/patentassignee/MD5_6bfdf49224a405be1f7b653c56ebad94 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12N5-10 |
| filingDate | 2018-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| inventor | http://rdf.ncbi.nlm.nih.gov/pubchem/patentinventor/MD5_29fb371a27bb98e0b81eeafdfef4a607 |
| publicationDate | 2020-02-06-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2020018193-A |
| titleOfInvention | Pluripotent stem cell detachment method |
| abstract | PROBLEM TO BE SOLVED: To provide a method for exfoliating pluripotent stem cells which excels in mass production, exfoliates pluripotent stem cells at a high survival rate, and can be recovered as a single cell. A method for exfoliating pluripotent stem cells cultured on a substrate, wherein the substrate has a layer made of a temperature-responsive polymer having a product of a layer thickness and a content ratio of a temperature-responsive component of 100 nm or less. And a method for exfoliating pluripotent stem cells, comprising the following steps [1] to [3]. [1] A step of contacting a pluripotent stem cell cultured on a substrate with an intercellular binding dissociation solution containing trypsin. [2] A step of removing the trypsin-containing cell-binding dissociation solution while the pluripotent stem cells adhere to the substrate. [3] A step of cooling the substrate and peeling the pluripotent stem cells from the substrate. [Selection diagram] None |
| priorityDate | 2018-07-31-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 275.