| abstract |
The present disclosure provides a CasX protein, a nucleic acid encoding the present CasX protein, and a modified host cell comprising the present CasX protein and / or a nucleic acid encoding the same. CasX protein is useful in various applications provided. The present disclosure relates to a CasX guide RNA that binds to and provides sequence specificity to the CasX protein, a nucleic acid encoding the CasX guide RNA, and a modified host comprising the CasX guide RNA and / or a nucleic acid encoding the same Provide cells. CasX guide RNA is useful in various applications provided. The present disclosure provides archaeal Cas9 polypeptides and nucleic acids encoding them, and their associated archaeal Cas9 guide RNAs and nucleic acids encoding them. |