http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019532623-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6818 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6869 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12Q1-6823 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6876 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-6869 |
| filingDate | 2017-09-04-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-11-14-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2019532623-A |
| titleOfInvention | Single nucleotide detection method and related probes |
| abstract | Methods are provided for sequencing nucleic acids, such as DNA or RNA. (1) generating a single nucleoside triphosphate stream by progressive enzymatic digestion of nucleic acids; (2) corresponding to at least one single nucleoside triphosphate in the presence of polymerase and ligase A biological probe, (a) a restriction endonuclease nicking site, a single nucleotide capture site for capturing a single nucleoside triphosphate, and an oligonucleotide region juxtaposed on either side of the nicking site, each A first single-stranded oligonucleotide, comprising at least one fluorophore and at least one quencher, such that the fluorophore is quenched, and (b) complementation with the first oligonucleotide Second and second capable of hybridizing on both sides of the capture site to a typical region Producing a used probe of at least one substantially double-stranded oligonucleotide by reacting with one comprising three single-stranded oligonucleotides; (3) a first of the used probes Nicking the oligonucleotide strands with a nicking restriction endonuclease at the nicking site to produce separate first oligonucleotide components, each with a fluorophore and quencher; (4) step (3 By separating the components of the first oligonucleotide produced in) from the complementary strand of the probe used, and (5) detecting the fluorophore in the components of the separated oligonucleotide with them. Characterized. This method is suitable for performing on microdroplets. New biological probes and probe systems for use with this method are also described. |
| priorityDate | 2016-09-02-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 260.