http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019530442-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-02827 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-0256 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-014 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N2291-02466 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N29-12 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N29-036 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N29-022 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N33-4833 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-04 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N5-06 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/G01N29-2443 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C12Q1-02 |
| filingDate | 2017-08-28-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-24-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2019530442-A |
| titleOfInvention | Real-time quantitative measurement of cell traction force |
| abstract | Placing AT-cut quartz and BT-cut quartz having the same frequency, surface morphology and / or modified with the same surface adhesion molecule into the culture dish or detection cell, and adding the cell to be tested to the culture dish or detection cell to adhere Alternatively, the cell traction force ΔS t at an arbitrary time t under various internal / external environmental stimuli is expressed by the equation ΔS t = (K AT −K BT ) −1 [tq AT Δf t AT / fr AT −tq BT Δf t BT / fr BT ] (wherein K AT = 2.75 × 10 −12 cm 2 dyn −1 and K BT = −2.65 × 10 −12 cm 2 dyn −1 are the stress coefficients of the AT cut crystal and the BT cut crystal, respectively. , fr AT the resonance frequency of the AT-cut crystal, fr BT is the resonant frequency of the BT-cut crystal, tq AT the thickness of the AT-cut quartz, tq BT is BT-cut crystal A thick, all real-time quantitative method of measuring cell traction and a step of measuring by a is) constant. Δf t AT and Δf t BT are frequency shifts at an arbitrary time t with respect to their reference points of the AT cut crystal and the BT cut crystal, respectively. The method can be used to study cell adhesion processes and dynamic changes in cell force under various internal and external environmental stimuli such as drug effects, drugs added before or after cell adhesion be able to. The method is applicable to all adherent cells including primary and passaged cells. [Selection] Figure 2 |
| priorityDate | 2016-08-29-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 82.