| abstract |
The present disclosure relates to, but is not limited to, a method of quantifying the amount of antibody molecules from a mixture comprising two or more antibody molecules, said method comprising said interaction by hydrophobic interaction chromatography high performance liquid chromatography (HIC-HPLC). Separating each of the two or more antibody molecules from a mixture and quantifying the amount of each antibody molecule, wherein the molecular weight of each antibody molecule is within 15 kDa of the other antibody molecules in the mixture Each antibody molecule differs from another antibody molecule in the mixture by more than about 0.25 units on the hydrophobicity index of Kite and Dolittle, or when analyzed alone by HIC-HPLC, Each is either or both eluting at different analysis times with little overlap with another antibody molecule in the mixture. |