| abstract |
The present disclosure provides a method for producing fXa derivative proteins on a large scale to obtain high yields of high purity protein products. In this method, a surfactant is added to a sample containing a polynucleotide construct encoding the protein, and the protein is analyzed via soybean trypsin inhibitor (STI) based affinity chromatography, ion exchange and mixed mode chromatography and hydrophobic interactions. May be purified. [Selection figure] None |