http://rdf.ncbi.nlm.nih.gov/pubchem/patent/JP-2019528238-A
Outgoing Links
| Predicate | Object |
|---|---|
| classificationCPCAdditional | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D2311-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D2315-16 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D2311-2626 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-145 |
| classificationCPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-24 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-327 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-362 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C07K1-36 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/C12N9-20 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D61-145 http://rdf.ncbi.nlm.nih.gov/pubchem/patentcpc/B01D15-1871 |
| classificationIPCInventive | http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-34 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-18 http://rdf.ncbi.nlm.nih.gov/pubchem/patentipc/C07K1-20 |
| filingDate | 2017-08-22-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationDate | 2019-10-10-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| publicationNumber | JP-2019528238-A |
| titleOfInvention | Method for purifying heterologous protein from egg white |
| abstract | The present invention relates to a method for purifying a heterologous protein from egg white. In some embodiments, the present disclosure relates to a method for purifying a heterologous protein from egg white comprising a heterologous protein, the method comprising: (a) adjusting the pH of the egg white to a pH of 5.8 to 6.5; Forming the adjusted egg white; (b) filtering the pH adjusted egg white of (a) and recovering the first filtrate; (c) hydrophobic interaction with the first filtrate of (b) Applying to the chromatography matrix and recovering the first eluate containing the heterologous protein; (d) adjusting the pH of the first eluate of (c) to pH 5.0 to 5.6, and adjusting the pH; Forming an eluate; (e) filtering the pH-adjusted eluate to obtain a second filtrate; (f) adjusting the pH of the second filtrate to pH 3.0 to 4.0; Forming an adjusted second filtrate; (g) pH adjustment of (f) Neutralizing the second filtrate to pH 5.0-8.0 to form a neutralized solution; (h) subjecting the neutralized solution to a cation exchange chromatography matrix and a second elution containing a heterologous protein. A step of collecting the liquid. |
| priorityDate | 2016-08-23-04:00^^<http://www.w3.org/2001/XMLSchema#date> |
| type | http://data.epo.org/linked-data/def/patent/Publication |
Incoming Links
Total number of triples: 387.